 |
| Name: |
HP 1050 HPLC System with Diode Array Detector |
| Item: |
#525 |
| Pirce: |
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| The system is in excellent condition and expertly refurbished. Includes Diode Array Detector, Quaternary Pump, 21-position Autosampler, Degasser and ONE (1) YEAR WARRANTY.
HP Chemstation Data System included with the purchase of the system and consists of computer, floppy drive, keyboard, mouse and HP G2170AA Chemstation software with license.
Diode Array Detector
| Detection Type |
Double beam photometer |
| Signals |
Up to 5, each defined by a sample wavelength and bandwidth and an optional reference wavelength and bandwidth. |
| Noise |
< ±2.0 x 10-5 AU peak-to-peak, at 254 nm with 4 nm bandwidth, flowing water at 1 ml/min, 1 second response time (10-90%) |
| Drift |
< 2 x 10-3 AU/hour, at 254 nm, after warm-up |
| Wavelength Range . |
190-600 nm, settable in 1 nm increments. |
| Bandwidth Range |
2-400 nm, settable in 1 nm increments. |
| Wavelength Accuracy |
±1 nm |
| Response Time |
8 choices, ranging from 0.1 to 20 seconds (10-90%). |
| Spectra |
Storage of at least 8 spectra, with definable wavelength range from 190-600 nm (total number of spectra depends on range defined). Post-run plotting of original or subtracted spectra possible. |
| Spectra Resolution |
Depends on slit width; |
| Absorbance Resolution |
< 5 x 10-6 AU |
| Spectra Acquisition |
12.5 ms from 190 to 600 nm; according to the responsetime settings scans are accumulated for one data point for improved sensitivity |
| Light Source |
Deuterium lamp. |
| Display |
2 line by 16 character fluorescent display with real-time display of operating parameters or absorbance and wavelength of maximum absorbance. |
| Methods |
Battery-backed storage of up to ten methods (depends on length of method), including initial and time-programmed parameters. Automatic startup and shutdown methods. Editing of stored methods possible during run from the workstation. |
| Analog Outputs |
Optional DAC board. If installed, two analog outputs are available for output of signals and/or signal combinations; both 1 V/2 AU or 100 mV/2 AU, user-selectable. |
Quaternary Pump
| Flow range |
Setpoints from 0.001 to 9.999 ml/min in 0.001 ml/min increments |
| Flow precision |
< 0.3% RSD (typically < 0.15 %), based on retention time at 0.5 ml/min and 2.5 ml/min |
| Pressure |
Operating range from 0-400 bar up to 5 ml/min; from 0-200 bar up to 10 ml/min |
| Compressibility compensation |
User-selectable, based on mobile phase compressibility |
| Recommended pH range |
2.3 to 12.5 |
| Gradient formation |
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve |
| Composition Range |
0 to 100% in 0.1% increments from four independent channels |
| Methods |
Battery-backed storage of up to 10 methods |
| Analog Output |
For pressure monitoring, 2 mV/bar |
Autosampler
| Injection range |
Programmable from 0.1 to 100 μl in 0.1 ul increments without hardware change required. Up to 1.8 ml with larger injection valve capillary. |
| Replicate Injections |
1-99 from one vial |
| Precision |
Typically < 0.5% RSD of peak areas from 5-100 μl and < 1% from 2-5 μl |
| Minimum Sample Volume |
1 ul can be sampled from 5 μl in 100 μl microvial or 10 μl in 300 μl microvial |
| Carryover |
< 0.1% typically |
| Sample Capacity |
21 or 34 in standard trays, with additional 100 in external tray (less two positions for access) |
| Injection Cycle Time |
20 seconds typically, depending on injection volume and drawing speed |
| Recommended pH Range |
2.3 to 9.5 standard |
| Methods |
Battery-backed storage of up to 10 methods. |
| Sequence Parameters |
Up to 10 parameter sets |
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